The Daily and Annual NO2 Concentrations for the Contiguous United States, 1-km Grids, v1 (2000-2016) data set contains daily predictions of Nitrogen Dioxide (NO2) concentrations at a high resolution (1 km x 1 km grid cells) for the years 2000 to 2016. An ensemble modeling framework was used to assess NO2 levels with high accuracy, which combined estimates from three machine learning models (neural network, random forest, and gradient boosting), with a generalized additive model. Predictor variables included NO2 column concentrations from satellites, land-use variables, meteorological variables, predictions from two chemical transport models, GEOS-Chem and the U.S. Environmental Protection Agency (EPA) Community Multiscale Air Quality Modeling System (CMAQ), along with other ancillary variables. The annual predictions were calculated by averaging the daily predictions for each year in each grid cell. The ensemble produced a cross-validated R-squared value of 0.79 overall, a spatial R-squared value of 0.84, and a temporal R-squared value of 0.73.